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Image Search Results
Journal: BioMed Research International
Article Title: Silencing of Plasma Membrane Ca 2+ -ATPase Isoforms 2 and 3 Impairs Energy Metabolism in Differentiating PC12 Cells
doi: 10.1155/2014/735106
Figure Lengend Snippet: The effect of PMCAs downregulation on complexes of respiratory chain. (a) The activity of ETC complexes I–III was assessed spectrophotometrically at 37°C in mitochondrial fraction and is expressed as nmol/min after normalization to citrate synthase activity as described in . (b) Complex IV activity was determined in digitonin-permeabilized cells using an oxygraph (Anton Paar) according to the procedure described in and is expressed as nmol O 2 /min after normalization to citrate synthase activity. The results are from n = 5 independent determinations. * P < 0.05 versus control cells. C: mock-transfected PC12, _2: PMCA2-reduced PC12, and _3: PMCA3-reduced PC12.
Article Snippet: Complex IV activity was determined in digitonin-permeabilized cells prepared as described in [ ], using an
Techniques: Activity Assay, Control, Transfection